High-Performance Computing at the NIH

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SpliceMap on Helix

SpliceMap is a de novo splice junction discovery and alignment tool. It offers high sensitivity and support for arbitrary RNA-seq read lengths.

SpliceMap is developed by Kin Fau, John Mu and collaborators at the Wong Lab, Stanford University

Program location

/usr/local/splicemap/bin

The easiest way to use the splicemap executables is to add them to your path using the modules commands, as in the example below.

biowulf% module load splicemap      (to add the splicemap executables to your path)

biowulf% module list                (if you want to see what modules you have loaded)
Currently Loaded Modulefiles:
  1) splicemap

How To Use

Example

helix% module load splicemap

helix% cd /data/user/mydir

helix% splicemap run1.cfg
where run1.cfg is the file that contains the splicemap configuration options for this run. A sample run.cfg file can be copied from /usr/local/splicemap/run.cfg. A set of sample data is available in /usr/local/splicemap/example.

 

Documentation

http://www.stanford.edu/group/wonglab/SpliceMap/